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A BioBrick Compatible Strategy for Genetic Modification of Plants.
Patrick Boyle, Devin R Burril, Mara C Inniss, Christina M Agapakis, Aaron Deardon, Jonathan DeWerd, Michael Geodeon, Jacqueline Quinn, Morgan Paull, Anugraha Raman, Mark Theilmann, Lu Wang, Julia Winn, Oliver Medvedik, Kurt Schellenberg, Karmella Haynes, Alain Viel, Tamara Brenner, George Church, Jagesh Shah, Pamela Silver
Journal of Biological Engineering
2012
Abstract
Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse creative input to the design process.This paper presents work done largely by undergraduate students participating in the 2010 International Genetically Engineered Machines (iGEM) competition. Described here is a framework for engineering the model plant Arabidopsis thaliana with standardized, BioBrick compatible vectors and parts available through the Registry of Standard Biological Parts (www.partsregistry.org). This system was used to engineer a proof-of-concept plant that exogenously expresses the taste-inverting protein miraculin.Our work is intended to encourage future iGEM teams and other synthetic biologists to use plants as a genetic chassis. Our workflow simplifies the use of standardized parts in plant systems, allowing the construction and expression of heterologous genes in plants within the timeframe allotted for typical iGEM projects.
